Feline Immunodeficiency Virus Matrix (MA) (FIV p15) (Internal Region) anticorps

N° du produit ABIN233823

N° du produit (Fournisseur): 600-401-992

Cet anticorps anti- est un anticorps Lapin Polyclonal détectant dans ELISA, WB et IHC. Adapté pour Feline Immunodeficiency Virus (FIV).

Aperçu rapide pour Feline Immunodeficiency Virus Matrix (MA) (FIV p15) (Internal Region) anticorps (ABIN233823)

Antigène: Feline Immunodeficiency Virus Matrix (MA) (FIV p15)

Reactivité: Feline Immunodeficiency Virus (FIV)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-992

Fournisseur: Rockland

Fonction: FIV matrix protein p15 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against FIV Matrix Protein p15.

Attributs du produit: Synonyms: rabbit anti-Feline immunodeficiency virus matrix protein p15 antibody, FIV Matrix Protein p15, Capsid protein p24, p1, Nucleocapsid protein p13, Gag polyprotein, Core polyprotein

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of FIV Matrix Protein p15.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting and suitable for immunocytochemistry.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 15 kDa in size corresponding to FIV Matrix Protein p15 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:10,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.22 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: Feline Immunodeficiency Virus Matrix (MA) (FIV p15)

Classe de substances: Viral Protein

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Feline immunodeficiency virus (FIV) belongs to the lentivirus family.  This family is characterized by assembly of the viral capsid at either the plasma membrane or at the limiting membrane of late endosomes.  The capsid assembles from the viral Gag polyprotein.  Upon release of a budding virion, Gag precursor protein is cleaved by the viral protease into its mature products, namely Matrix Protein, Capsid and Nucleocapsid.  Matrix Protein, located at the N-terminus of the Gag polyprotein, is usually myristylated during protein translation, prior to the later events of virus assembly.  The myristate moiety is believed to be sequestered within the Matrix Protein during protein translation and later facilitates membrane binding upon exposure resulting from conformational changes.  Essential functions attributed to the Matrix Protein of lentiviruses include targeting newly synthesized Gag precursor proteins to the site of virus assembly by binding with cellular components such as phosphatidylinositides.  In the mature virus particle, the Matrix Protein provides internal structure to the virion within the capsid, but is not exposed at the surface of the particle.  Based on studies with HIV, it is postulated that FIV Matrix Protein may also serve additional functions, including nuclear localization of the viral core upon entry of the virus into a new host cell.

ID gène: 1489988, 120815

UniProt: P16087